This is an optional step. Each web application uses a specific application pool, which uses a specific Windows account.
The Windows account used by the application pool can be changed as follows:
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Click the Application Pools node and right-click the application pool whose name matches the virtual directory name of the Portal (for example, Portal).
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Select Advanced Settings and open the Identity setting.
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Select Custom Account and Set the credentials that you want to use.
Figure 1. IIS Manager - Change SQL Server account access